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Literature summary extracted from
- A phosphate-binding subsite in bovine pancreatic ribonuclease A can be converted into a very efficient catalytic site (2007), Protein Sci., 16, 99-109.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.6.1.18 | H12K/H119Q | 0.007% of wild-type activity | Bos taurus |
| 4.6.1.18 | K7H/R10H | 17% of wild-type activity, introduction of a putative new catalytic site resulting in increase in exonucleolytic activity | Bos taurus |
| 4.6.1.18 | K7H/R10H/H12K/H119Q | 9% of wild-type activity due to suppression of native active site, increase in exonucleolytic activity | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.6.1.18 | 0.0023 | - |
cyclic 2',3'-cytidine monophosphate | wild-type, pH 5.5, 25°C | Bos taurus |  |
| 4.6.1.18 | 0.0026 | - |
cyclic 2',3'-cytidine monophosphate | mutant K7H/R10H, pH 5.5, 25°C | Bos taurus | |
| 4.6.1.18 | 0.015 | - |
pentacytidylic acid | mutant K7H/R10H/H12K/H119Q, pH 7.0 | Bos taurus | |
| 4.6.1.18 | 0.028 | - |
pentacytidylic acid | mutant K7H/R10H, pH 7.0 | Bos taurus | |
| 4.6.1.18 | 0.038 | - |
pentacytidylic acid | wild-type, pH7.0 | Bos taurus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.6.1.18 | Bos taurus | P61823 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.18 | cyclic 2',3'-cytidine monophosphate + H2O | - |
Bos taurus | 3'-CMP | - |
? | |
| 4.6.1.18 | pentacytidylic acid + H2O | - |
Bos taurus | ? | - |
? | |
| 4.6.1.18 | poly(C) + H2O | - |
Bos taurus | 3'-CMP + 3'-phospho-oligo(C) | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.6.1.18 | 3.5 | - |
cyclic 2',3'-cytidine monophosphate | mutant K7H/R10H, pH 5.5, 25°C | Bos taurus | |
| 4.6.1.18 | 12 | - |
cyclic 2',3'-cytidine monophosphate | wild-type, pH 5.5, 25°C | Bos taurus | |
| 4.6.1.18 | 27 | - |
pentacytidylic acid | mutant K7H/R10H/H12K/H119Q, pH 7.0 | Bos taurus | |
| 4.6.1.18 | 54 | - |
pentacytidylic acid | mutant K7H/R10H, pH 7.0 | Bos taurus | |
| 4.6.1.18 | 323 | - |
pentacytidylic acid | wild-type, pH 7.0 | Bos taurus | |
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Release 2023.1 (January 2023)
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